《组织学技术》自1977年第一版问世以来，历经修订，已成为全世界组织学家，病理学家的重要工具书。《组织学技术》内容全面，涵盖了组织学技术的方方面面，既包括传统的常用技术，又介绍了近年来新发展的、有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术；对于结缔组织、糖类，脂类、蛋白质．核酸、色素及矿物质、淀粉、神经内分泌颗粒。微体、骨。神经系统等相关的组织学技术也予专门章节讲述；另外还涉及了显微镜样品定量，实验室安全及管理，以及人体工程学等相关内容。既介绍各技术的理论、机制，又讲述其应用价值，特别是对有重要诊断意义的技术予以重点论述，凸现了组织学技术对临床实践的意义。
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目录
List of contributors
Preface
Preface to the first edition
General acknowledgments
AcknowIedgment to Alan Stevens
1.Managing the Laboratory
Marilyn Gamble,lain Banks and John D．Bancroft
2.Safety in the Laboratory
Richard W．Dapson
3.Light Microscopy
John D．Bancroft and Alton D．Floyd
4.Fixation of Tissues
William E．Grizzle，Jerry L．Fredenburgh and Russell B．Myers
5.The Gross Room/Surgical Cutup
Paul E．Billings and William E．Grizzle
6.Tissue Processing
LenaT．Spencer and John D．Bancroft
7.Microtomy：Paraffin and Frozen
Lena T．Spencer and John D．Bancroft
8.How do Histological Stains Work?
Richard W．Horobin
9.The Hematoxy 8ins and Eosin
Marilyn Gamble
10.Connective Tissues and Stains
M．Lamar Jones,John D.Bancroft and Marilyn Gamble
11.Carbohydrates
Russell B．Myers，Jerry L．Fredenburgh and William E．Grizzle
12.Lipids
M．Lamar Jones
13.Proteins and Nudeic Acids
Jerry L．Fredenburgh，John D．Bancroft,William E．Grizzle and Russell B．Myers
14.Pigments and Minerals
Charies J．Churukian
15.Amyloid
Geoffrey H．Vowles
16.The Dispersed Neuroendocrine System,Cytoplasmic Granules and other Organelles
William E．Grizzle and John D．Bancroft
17.Microorganisms
Jeanine H．Bartlett
18.Bone
Gayle M．Callis
19.Techniques in Neuropathology
Scott L．Nestor
20.Enzyme Histachemistry and its Diagnostic Applications
Scott L．Nestor and John D．Bancroft
21.lmmunohistochemical Techniques
Peter Jackson and David Blythe
22.lmmunohistochemistry Quality Control
Christa L．Hladik and Charles L．White
23.lmmunohistochemistry Applications in Pathology
Charles L．White
24.lmmunofluorescent Techniques
Christa L．Hladik and Charles L．White
25.Tissue Microarray
Wanda Grace-Jones
26.Molecular Pathology--In Situ hybridization
Dlane L．Sterchi
27.Genetic Testing：Utilization of Fluorescent In Situ Hybridization(FISH)
Caroline Astbury
28.Laser Micodissection
Diane L．Sterchi
29.Plastic Embedding for Light Microscopy
Neil M．Hand
30.Electron Microscopy
Anthony E．Woods and John w．StMing
31.Quantitative Data from Microscopic Specimens
Alton D．Floyd
32.Ergonomics
Janet，Minshew
Appendices
William E．Grizzle，Jerry L．Fredenburgh and Russell B．Myers
Staining Methods Index
Subject Index
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